bs-3031R

antibody from Bioss

Targeting: MAP3K5 ASK1, MAPKKK5, MEKK5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-3031R

Provider

Bioss

Proper citation

Bioss Cat#bs-3031R, RRID:AB_10856323

Product name

ASK1 (Thr845) Polyclonal Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic phosphopeptide derived from mouse ASK1 around the phosphorylation site of Thr845

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

References

Submitted references

Scutellarein suppresses Aβ-induced memory impairment via inhibition of the NF-κB pathway in vivo and in vitro.

Huang XW, Xu Y, Sui X, Lin H, Xu JM, Han D, Ye DD, Lv GF, Liu YX, Qu XB, Duan MH
Oncology letters 2019 Jun;17(6):5581-5589

---

Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.

Yang Z, Zhang C, Feng Y, Qi W, Cui Y, Xuan Y
Experimental and molecular pathology 2019 Aug;109:104239

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

L1 rat heart, L2 rat brain lysates probed (bs-3031R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted and observed band size: 155kDa.

Sample type

Rat

Other comments

Heart

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-phospho-ASK1(Thr845) Polyclonal Antibody, Unconjugated (bs-3031R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining

Sample type

Rat

Other comments

Heart