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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- Ab089824 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- ASK1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human ASK1 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.Application：WB: 1/500-1/2000IHC: 1/50-1/100Protein Function：Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1001-1156- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ASK1 Antibody (Ab089824) - Western Blot All lanes: ASK1 Antibody (Ab089824) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 155 kDa Observed band size: 154 kDa Exposure time: 180.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ASK1 Antibody (Ab089824) - Western Blot All lanes: ASK1 Antibody (Ab089824) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 155 kDa Observed band size: 154 kDa Exposure time: 180.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ASK1 Antibody (Ab089824) - Western Blot All lanes: ASK1 Antibody (Ab089824) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 155 kDa Observed band size: 154 kDa Exposure time: 47.2 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ASK1 Antibody (Ab089824) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using ASK1 Antibody (Ab089824). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
