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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-32863 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin E2 Monoclonal Antibody (40-89)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 40-89
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclin E2 in Hela cell lysate using a Cyclin E2 Monoclonal antibody (Product # MA5-32863).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Cyclin E2 Monoclonal Antibody (40-89) (Product # MA5-32863) and a 41 kDa band corresponding to G1/S-specific cyclin-E2 was observed across the tissues tested except Mouse Heart, Mouse Liver, Mouse Lung, Rat Hear and Mouse Kidney. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Heart (Lane 2), Rat Brain (Lane 3), Mouse Liver (Lane 4), Mouse Lung (Lane 5), Rat Heart (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Cyclin E2 in Hela cells using a Cyclin E2 Monoclonal antibody (Product # MA5-32863)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cyclin E2 of paraffin-embedded Human breast carcinoma tissue using a Cyclin-E2 Monoclonal antibody (Product #MA5-32863).
