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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10197 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10197, RRID:AB_1671803
- Product name
- Ccne2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Ccne2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cdc2-cyclin E complexes regulate the G1/S phase transition.
Mammalian cell cycles without cyclin E-CDK2.
Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells.
Aleem E, Kiyokawa H, Kaldis P
Nature cell biology 2005 Aug;7(8):831-6
Nature cell biology 2005 Aug;7(8):831-6
Mammalian cell cycles without cyclin E-CDK2.
Yu Q, Sicinski P
Cell cycle (Georgetown, Tex.) 2004 Mar;3(3):292-5
Cell cycle (Georgetown, Tex.) 2004 Mar;3(3):292-5
Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells.
Parisi T, Beck AR, Rougier N, McNeil T, Lucian L, Werb Z, Amati B
The EMBO journal 2003 Sep 15;22(18):4794-803
The EMBO journal 2003 Sep 15;22(18):4794-803
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Ccne2 polyclonal antibody (Cat # PAB10197) shows specific detection of Ccne2.Cell extracts over-expressing mouse Ccne1 (Lane 1) and Ccne2 (Lane 2) were electrophoresed, transferred to nitrocellulose, and probed with theCcne2 polyclonal antibody.The affinity purified antibody also detects endogenous Ccne2 in Skp2 -/- MEF cells. (data not shown).Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
