- MA3-16771 - Invitrogen Antibodies FBL antibody

Submitted references Complete loss of H3K9 methylation dissolves mouse heterochromatin organization.

Montavon T, Shukeir N, Erikson G, Engist B, Onishi-Seebacher M, Ryan D, Musa Y, Mittler G, Meyer AG, Genoud C, Jenuwein T
Nature communications 2021 Jul 16;12(1):4359

H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.

Bacci L, Aiello A, Ripoli C, Loria R, Pugliese D, Pierconti F, Rotili D, Strigari L, Pinto F, Bassi PF, Mai A, Grassi C, Pontecorvi A, Falcioni R, Farsetti A, Nanni S
International journal of molecular sciences 2019 Aug 17;20(16)

Optimized protocol for combined PALM-dSTORM imaging.

Glushonkov O, Réal E, Boutant E, Mély Y, Didier P
Scientific reports 2018 Jun 8;8(1):8749

Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer.

Re A, Colussi C, Nanni S, Aiello A, Bacci L, Grassi C, Pontecorvi A, Farsetti A
Oncotarget 2018 Jun 15;9(46):27985-27997

Olfactory memory is enhanced in mice exposed to extremely low-frequency electromagnetic fields via Wnt/β-catenin dependent modulation of subventricular zone neurogenesis.

Mastrodonato A, Barbati SA, Leone L, Colussi C, Gironi K, Rinaudo M, Piacentini R, Denny CA, Grassi C
Scientific reports 2018 Jan 10;8(1):262

40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.

Missbach S, Weis BL, Martin R, Simm S, Bohnsack MT, Schleiff E
PloS one 2013;8(1):e54084

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of yeast protein extracts stained with Product # MA3-16771 (Lane 1), showing a single band at ~34kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fibrillarin in lysates of cell fractions. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA3-16771 using a dilution of 1:500. Antibody in green: [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37 kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fibrillarin in lysates of cell fractions. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA3-16771 using a dilution of 1:500. Antibody in green: [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37 kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fibrillarin in yeast protein extracts. Sample was incubated in Fibrillarin monoclonal antibody (Product # MA3-16771).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Fibrillarin was achieved by transfecting HeLa cells with Fibrillarin specific siRNAs (Silencer® select Product # s4822, s4820). Western blot analysis (Fig. a) was performed using whole cell extracts from the Fibrillarin knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Fibrillarin Monoclonal Antibody (Product # MA3-16771, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fibrillarin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) extracts (30 µg lysate) of HeLa (Lane 1), HepG2 (Lane 2), HT-29 (Lane 3), MCF-7 (Lane 4), U-2 OS (Lane 5), A-431 (Lane 6), NIH-3T3 (Lane 7), PC-12 (Lane 8) and PANC-1 (Lane 9). The blot was probed with Fibrillarin Monoclonal Antibody (38F3) (Product # MA3-16771, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 39 kDa band corresponding to Fibrillarin was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Fibrillarin in HeLa cells. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA3-16771) using a dilution of 1:100. Antibody from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Fibrillarin in MCF7 cells. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA3-16771) followed by Alexa Fluor 488-conjugated Goat to mouse IgG secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Fibrillarin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fibrillarin Monoclonal Antibody (38F3) (Product # MA3-16771) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleolar localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Fibrillarin in human ovarian cancer. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA3-16771) followed by DAB with hematoxylin counterstain.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Fibrillarin in HEK293 cells. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA3-16771) using a dilution of 1:400 followed by Alexa Fluor 488 secondary. Secondary (shown in green) alongside unstained cells (shown in red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Confocal microscopy imaging of nucleolar sub-domains in HeLa cells. Column 1: NPM protein fused to the fluorescent protein eGFP was overexpressed after cell transient transfection in order to image the nucleoli granular component ( A1 ). Endogenous RPA was immunolabelled with A647-Ab ( B1 ). The merged images evidenced the specificity of the labelling strategy ( C1 ). Column 2: eGFP-NPM localization is displayed in panel A2. Endogenous Fib was immunolabelled with A647-Ab ( B2 ). The merged images evidenced the spatial overlap between the two domains ( C2 ). Column 3: Endogenous Fib was immunolabelled with A555-Ab ( A3 ). Endogenous RPA was immunolabelled with A647-Ab ( B3 ). The merged images evidenced the spatial overlap between the two domains ( C3 ). Scale bar: 5 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 eNOS binds Nup153 promoter and regulates its expression in an estrogen-dependent manner (A) Integrated Genome Viewer (IGV 2.3) screenshot showing peaks of eNOS identified by ChIP-Seq at the genomic regions encoding Nup153 in PCa cells (C27IM) in the absence (NT) or presence of 17beta-estradiol (E 2 , 10 -7 M, 45min). Region amplified in panel B is indicated with a red circle. (B) Recruitment of eNOS and ERbeta on the promoter region of Nup153 by ChIPs in the presence or absence of E 2 (45 min) in C27IM cells. The immunoprecipitations were performed using anti-eNOS (Type III, BD), anti-ERbeta (L-20) or no antibody (NoAb) as a negative control. Values are represented as fold of induction (+/-E 2 ) and as mean +/-SEM of 3 independent experiments. * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Nup153 forms complexes with eNOS, ERbeta and p300 (A) Confocal analysis of Prostate Cancer (PCa, C27IM) and Benign Prostatic Hyperplasia (BPH, C17IM) cells stained with antibodies to Nup153 ([QE5]; red) or eNOS (Type III; green). Scale bar: 20mum (Nup153, eNOS and MERGE; zoomed area is showed). (B) Analysis of Nup153 interaction with eNOS by Co-Immunoprecipitation in PCa and BPH cells in basal condition. Immunoprecipitation with IgG served as negative control. (C) Analysis of Nup153 interaction with eNOS and ERbeta by Co-Immunoprecipitation in PCa cells in basal condition or upon E 2 treatment (10 -7 M, 24h). PCa cells were immunoprecipitated with Nup153 Antibody ([7AB], Abcam) or with ERbeta (GeneTex, #110607). Immunoprecipitation with IgG served as negative control. Membranes were blotted with specific antibodies to Nup153 ([Q5], Abcam), ERbeta (GeneTex, #110607) and eNOS (Type III, BD). H1 served as control. 4mug of transfected HeLa with human ERbeta short isoform (485aa) were used as positive control for Estrogen Receptor. Total proteins were resolved by SDS-PAGE using a 10% Invitrogen precast gel (NuPage and MES buffer). (D) Analysis of Nup153 interaction with eNOS and ERbeta by Co-Immunoprecipitation in HeLa cells transfected with empty vector (PSG5) or with human ERbeta full-length (ERbeta 530aa), before and after E 2 treatment (10 -7 M, 24h). HeLa cells were immunoprecipitated with Nup153 Antibody ([7AB], Abcam). Immunoprecipitation with IgG served as negativ

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 Collapse of heterochromatin in compound H3K9 KMT-mutant MEF cells. a DNA FISH for major satellite repeats (MSR) in control and compound H3K9 KMT mutant. Percentages indicate cells that display the shown pattern. N = 501 cells examined for WT, 303 for E1 , 352 for E1 S1S2 , 304 for 6KO1 , 308 for E2 , 311 for E1E2 , 301 for E1E2 G9Gl , and 309 for 6KO2 over three independent experiments. For each image, lower panels show DAPI counterstaining of the same nuclei. b Immunofluorescence for Lamin B, marking the nuclear lamina. Percentages indicate cells that display the shown pattern. N = 680 cells examined for WT, 785 for E1 , 302 for E1 S1S2 , 305 for 6KO1 , 573 for E2 , 499 for E1E2 , 558 for E1E2 G9Gl, and 427 for 6KO2 over three independent experiments. For each image, lower panels show DAPI counterstaining of the same nuclei. c Immunofluorescence for Fibrillarin, marking nucleoli. Representative images ( N = 100 cells) are shown. For each image, lower panels show DAPI counterstaining of the same nuclei. Scale bars represent 5 mum.

MA3-16771

Antibody data