- PA5-29183 - Invitrogen Antibodies CAT antibody

Submitted references β-Nicotinamide Mononucleotide (NMN) Administrated by Intraperitoneal Injection Mediates Protection Against UVB-Induced Skin Damage in Mice.

Zhou X, Du HH, Long X, Pan Y, Hu J, Yu J, Zhao X
Journal of inflammation research 2021;14:5165-5182

Ketogenic diet regulates the antioxidant catalase via the transcription factor PPARγ2.

Knowles S, Budney S, Deodhar M, Matthews SA, Simeone KA, Simeone TA
Epilepsy research 2018 Nov;147:71-74

PDGF-BB Preserves Mitochondrial Morphology, Attenuates ROS Production, and Upregulates Neuroglobin in an Astrocytic Model Under Rotenone Insult.

Cabezas R, Vega-Vela NE, González-Sanmiguel J, González J, Esquinas P, Echeverria V, Barreto GE
Molecular neurobiology 2018 Apr;55(4):3085-3095

Nonalcoholic steatohepatitis severity is defined by a failure in compensatory antioxidant capacity in the setting of mitochondrial dysfunction.

Boland ML, Oldham S, Boland BB, Will S, Lapointe JM, Guionaud S, Rhodes CJ, Trevaskis JL
World journal of gastroenterology 2018 Apr 28;24(16):1748-1765

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Catalase using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Catalase using 30 µg of A) Neuro2A (B) GL261 and C) C8D30 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Catalase using 50 µg of rat kidney lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), K-562 (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Rat Liver (Lane 4). The blot was probed with Anti-Catalase Polyclonal Antibody (Product # PA5-29183, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 59 kDa band corresponding to Catalase was observed across cell lines and tissues tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Catalase was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Catalase Polyclonal Antibody (Product # PA5-29183) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Catalase was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Catalase Polyclonal Antibody (Product # PA5-29183) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Catalase Polyclonal Antibody (Product # PA5-29183). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase Polyclonal Antibody (Product # PA5-29183) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Catalase was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Catalase Polyclonal Antibody (Product # PA5-29183) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Catalase (Product # PA5-29183) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Protein expression of AMPK, NFkappaB-p65, IkappaB-alpha, SOD1 and CAT in skin tissues. ( A ) relative expression levels of proteins; ( B ) protein banding map. * p < 0.05 compared to the UVB group; ** p < 0.01 compared to the UVB group; *** p < 0.001 compared to the UVB group. Abbreviations : VC+UVB, mice treated with vitamin C(300mg/kg) and UVB irradiation; NMN+UVB, mice treated with nicotinamide mononucleotide (300mg/kg) and UVB irradiation.

PA5-29183

Antibody data