Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 651201 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#651201, RRID:AB_10900815
- Product name
- Purified anti-ATM Phospho (Ser1981)
- Antibody type
- Monoclonal
- Antigen
- KLH conjugated synthetic peptide SLAFEEGSpQSTTISS
- Description
- Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. ATM is held inactive in unirradiated cells as a dimer or higher-order multimer. Cellular irradiation induces rapid intermolecular autophosphorylation of serine 1981 that causes dimer dissociation and initiates cellular ATM kinase activity. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-ATM pS1981 (clone 10H11.E12) antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1 - lysates from untreated Hela cells; Lane 2 - lysates from Hela cells treated with UV.
- Conjugate
- Unconjugated