- MA1-2020 - Invitrogen Antibodies ATM antibody

Wang C, Xu W, Zhang Y, Zhang F, Huang K
Cell death & disease 2018 Oct 15;9(11):1047

van Sluis M, McStay B
Genes & development 2015 Jun 1;29(11):1151-63

Schweikl H, Petzel C, Bolay C, Hiller KA, Buchalla W, Krifka S
Biomaterials 2014 Mar;35(9):2890-904

Bakkenist CJ, Drissi R, Wu J, Kastan MB, Dome JS
Cancer research 2004 Jun 1;64(11):3748-52

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of phospho-ATM (pSer1981) was performed using phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020) on gamma-irradiated HeLa cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of phospho-ATM (pSer1981) was performed by loading 40 µg of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST (Product # 37525) for at least 1 hour. The membrane was probed with a phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ATM phosphorylated on pSer1981 (green) in HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020), at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) (top panels). A merged image with nuclei staining (blue) using Hoechst 33342 dye (Product # 62249) is shown on bottom panels. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Phospho-ATM pSer1981 in 293 untreated cells (left panel) or stimulated cells with 100 mJ UV (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-ATM pSer1981 Monclonal Antibody (10H11) (Product # MA1-2020) at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Phospho-ATM pSer1981 (green) showing staining in HEK-293 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-ATM pSer1981 monoclonal antibody (Product # MA1-2020) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Phospho-ATM pSer1981 was performed using 70% confluent log phase HeLa cells irradiated with UV for 4 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-ATM (Ser 1981) (10H11) Mouse Monoclonal Antibody (Product # MA1-2020) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the untreated cells. Panel f represents the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

MA1-2020