AF1655

antibody from R&D Systems

Targeting: ATM ATA, ATC, ATD, ATDC, TEL1, TELO1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1655

Provider

R&D Systems

Product name

Human/Mouse/Rat Phospho-ATM (S1981) Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human ATM when phosphorylated at S1981. Also detects the comparable phosphorylated sites in mouse ATM (S1987) and rat ATM (S1952).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

ATM directs DNA damage responses and proteostasis via genetically separable pathways.

Lee JH, Mand MR, Kao CH, Zhou Y, Ryu SW, Richards AL, Coon JJ, Paull TT
Science signaling 2018 Jan 9;11(512)

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DEK is required for homologous recombination repair of DNA breaks.

Smith EA, Gole B, Willis NA, Soria R, Starnes LM, Krumpelbeck EF, Jegga AG, Ali AM, Guo H, Meetei AR, Andreassen PR, Kappes F, Vinnedge LM, Daniel JA, Scully R, Wiesmüller L, Wells SI
Scientific reports 2017 Mar 20;7:44662

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PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination.

Clarke TL, Sanchez-Bailon MP, Chiang K, Reynolds JJ, Herrero-Ruiz J, Bandeiras TM, Matias PM, Maslen SL, Skehel JM, Stewart GS, Davies CC
Molecular cell 2017 Mar 2;65(5):900-916.e7

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DNA-PK-A candidate driver of hepatocarcinogenesis and tissue biomarker that predicts response to treatment and survival.

Cornell L, Munck JM, Alsinet C, Villanueva A, Ogle L, Willoughby CE, Televantou D, Thomas HD, Jackson J, Burt AD, Newell D, Rose J, Manas DM, Shapiro GI, Curtin NJ, Reeves HL
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Feb 15;21(4):925-33

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Direct activation of ATM by resveratrol under oxidizing conditions.

Lee JH, Guo Z, Myler LR, Zheng S, Paull TT
PloS one 2014;9(6):e97969

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Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.

Jurk D, Wilson C, Passos JF, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender SL, Fullard N, Nelson G, Mann J, van de Sluis B, Mann DA, von Zglinicki T
Nature communications 2014 Jun 24;2:4172

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Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia.

Byrd PJ, Srinivasan V, Last JI, Smith A, Biggs P, Carney EF, Exley A, Abson C, Stewart GS, Izatt L, Taylor AM
British journal of cancer 2012 Jan 17;106(2):262-8

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Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours.

Reiman A, Srinivasan V, Barone G, Last JI, Wootton LL, Davies EG, Verhagen MM, Willemsen MA, Weemaes CM, Byrd PJ, Izatt L, Easton DF, Thompson DJ, Taylor AM
British journal of cancer 2011 Aug 9;105(4):586-91

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RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine.

Rajesh P, Rajesh C, Wyatt MD, Pittman DL
DNA repair 2010 Apr 4;9(4):458-67

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Inhibition of CHK1 kinase by Gö6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells.

Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT
Biochemical pharmacology 2009 Mar 1;77(5):770-80

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Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.

Leonard JM, Ye H, Wetmore C, Karnitz LM
The Journal of cell biology 2008 Nov 3;183(3):385-91

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat Phospho-ATM (S1981) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase ( lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Phospho-ATM (S1981) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.