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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 55080 - Provider product page
- Provider
- AnaSpec
- Proper citation
- AnaSpec; EGT Group Cat#55080, RRID:AB_2134825
- Product name
- Anti-Keratin-14 (IN) antibody
- Antibody type
- Polyclonal
- Description
- This rabbit polyclonal antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 ml of 1 x Phosphate Buffered Saline (PBS, pH 7.5) with 0.05% Sodium Azide as preservative.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.2 mg/ml
- Storage
- 4°C
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Supportive validation
- Submitted by
- AnaSpec (provider)
- Main image
- Experimental details
- Figure 3. Western blot of keratin 14 in A431 cell lysate labeled with the Anti-Keratin-14 (IN) antibody (Cat# 55080) at 1:200 dilution. An immunoreactive band at ~52 kDa was detected.
Supportive validation
- Submitted by
- AnaSpec (provider)
- Main image
- Experimental details
- Figure 1. IHCs of FFPE human breast tissue sections probed with Anti-Keratin-14 (IN) antibody, Cat# 55080 at 1:150 dilution. Antigen retrieval was accomplished by using 0.01% pepsin diluted in HCl, pH 2, incubated for 30 min at 37 in a humidified chamber. Secondary antibody used was donkey anti-rabbit labeled with Alexa-488 (Invitrogen), and nuclei were stained with DAPI. (Images courtesy of Dr. Andra Frost, Univ. of Alabama at Birmingham).
