Explore
Validate
LearnPA5-49749
antibody from Invitrogen Antibodies
Targeting: CDKN2B
CDK4I, INK4B, MTS2, P15, p15INK4b, TP15
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-49749 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDKN2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total CDKN2B protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Long non-coding RNA ANRIL alleviates H(2)O(2)-induced injury by up-regulating microRNA-21 in human lens epithelial cells.
Du S, Shao J, Qi Y, Liu X, Liu J, Zhang F
Aging 2020 Apr 20;12(8):6543-6557
Aging 2020 Apr 20;12(8):6543-6557
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDKN2B in extracts from 293 cells (Lane 2). Samples were probed using CDKN2B antiobdy (Product # PA5-49749).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDKN2B in extracts from HeLa cells. Samples were probed using CDKN2B antibody (Product # PA5-49749).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDKN2B in HeLa cells. Samples were probed using a CDKN2B polyclonal antibody (Product # PA5-49749).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CDKN2B in paraffin-embedded Human lung carcinoma tissue using CDKN2B Polyclonal Antibody (Product # PA5-49749).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 LncRNA ANRIL attenuated H 2 O 2 -induced HLEC injury. HLEC SRA01/04 cells were transfected with pcDNA3.1 or pc-ANRIL, and untransfected cells were acted as control. ( A ) Expression of lncRNA ANRIL was determined by RT-qPCR. Transfected or untransfected SRA01/04 cells were treated with 400 muM H 2 O 2 for 1 h, and non-treated cells were acted as control. ( B ) Cell viability was measured by CCK-8 assay. ( C , D ) Expression of p53, cyclinD1 and CDK4 was testified by Western blot analysis. ( E , F ) Percentage of apoptotic cells was quantified by flow cytometry assay. ( G ) Expression of proteins related to apoptosis and ( I ) p14, p15 and p19 were detected by Western blot analysis. ( H ) gammaH2AX staining for detection of DNA levels. Data are shown as the mean +- SD of three independent experiments. *, P < 0.05; ***, P < 0.001.
