16815-1-AP

antibody from Invitrogen Antibodies

Targeting: BCAR1 CAS, CASS1, Crkas, P130Cas

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16815-1-AP

Provider

Invitrogen Antibodies

Product name

p130Cas Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNHLNVLAK ALYDNVAESP DELSFRKGDI MTVLEQDTQG LDGWWLCSLH GRQGIVPGNR LKILVGMYDK KPAGPGSGPP ATPAQPQPGL HAPAPPASQY TPMLPNTYQP QPDSVYLVPT PSKAQQGLYQ VPGPSPQFQS PPAKQTSTFS KQTPHHPFPN PATDLYQVPP GPGGPAQDIY QVPPSAGMGH DIYQVPPSMD TRSWEGTKPP AKVVVPTRVG QGYVYEAAQP EQDEYDIPRH LLAPGPQDIY DVPPVRGLLP SQYGQEVYDT PPMAVKGPNG RDPLLEVYDV PPSVEKGLPP SNHHAVYDVP PSVSKDVPDG PLLREETYDV PPAFAKAKPF DPARTPLVLA APPPDSPPAE DVYDVPPPAP DLYDVPPGLR RPGPGTLYDV PRERVLPPEV ADGGVVDSGV YAVPPPAERE A (1-420 aa encoded by BC062556)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using BCAR1 antibody 16815-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 16815-1-AP (p130Cas antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 16815-1-AP (p130Cas antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 A431 cells were stained with 0.2 µg Anti-Human p130Cas (Product # 16815-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), or stained with 0.2 µg isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (black). Cells were fixed with 90% MeOH.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-p130Cas (IP: 16815-1-AP, 4 µg; Detection: 16815-1-AP 1:600) with RAW 264.7 cells lysate 4000 µg.