MA5-14276

antibody from Invitrogen Antibodies

Targeting: BCAR1 CAS, CASS1, Crkas, P130Cas

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14276

Provider

Invitrogen Antibodies

Product name

p130Cas Monoclonal Antibody (CAS-14 (35B-1A4))

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14276 targets p130Cas in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples. The MA5-14276 immunogen is recombinant human p130Cas protein.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

CAS-14 (35B-1A4)

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Ezrin and BCAR1/p130Cas mediate breast cancer growth as 3-D spheroids.

Konstantinovsky S, Davidson B, Reich R
Clinical & experimental metastasis 2012 Aug;29(6):527-40

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Breast carcinoma cells in primary tumors and effusions have different gene array profiles.

Konstantinovsky S, Smith Y, Zilber S, Tuft Stavnes H, Becker AM, Nesland JM, Reich R, Davidson B
Journal of oncology 2010;2010:969084

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Proteomic analysis of selected prognostic factors of breast cancer.

Roberts K, Bhatia K, Stanton P, Lord R
Proteomics 2004 Mar;4(3):784-92

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of p130Cas using p130Cas Monoclonal Antibody (Product # MA5-14276) on HUVEC Cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human breast cancer stained with p130 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded rat breast cancer stained with p130 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of p130Cas using p130Cas Monoclonal Antibody (Product # MA5-14276) on denatured Human HUVEC Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Validation by immunohistochemistry . (a)-(b), p130cas: Immunostaining for p130cas in a primary carcinoma (a) and pleural effusion (b) showing cytoplasmic staining in tumor cells. (c)-(f), p-Ezrin: Immunostaining for p-Ezrin in a primary carcinoma (c), pleural effusion from the same patient (d), and 2 additional effusions obtained from the pericardial (e) and pleural (f) cavity. Tumor cells in the primary carcinoma are p-Ezrin-negative, whereas cells in all three effusions are stained at the membrane. (g)-(i), claudin-4: immunostaining for Claudin-4 in a primary carcinoma (g), pleural effusion from the same patient (h), and an additional effusion obtained from the pericardial cavity (i, same specimen as in e). Tumor cells in the primary carcinoma are Claudin-4 -negative, whereas cells in the two effusions are stained at the membrane.