LS-C142009

antibody from LSBio

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C142009

Provider

LSBio

Product name

Anti-CDKN2A / p16INK4a Antibody (aa54-75, clone 5-C3-1, DY650) LS-C142009

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Mouse

Host

Rat

Conjugate

Red dye

Isotype

IgG

Antibody clone number

5-C3-1

Vial size

100µl

Storage

Store at 4°C. Do not freeze.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of endogenous p19Arf using LS-C2744. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (lane 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (lane 2), were resolved by SDS-PAGE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of p19Arf using LS-C2744. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or LS-C2744 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical staining of sections from a wild type mouse testis taken at P11 were probed with LS-C2744 (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Human Liver (formalin-fixed, paraffin-embedded) stained with CDKN2A antibody LS-B200 at 20 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.