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LearnMAB2416
antibody from Abnova Corporation
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
Reference
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- Product number
- MAB2416 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2416, RRID:AB_10628308
- Product name
- Cdkn2a monoclonal antibody, clone 12-A1-1
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide corresponding to amino acids 1-100 of mouse Cdkn2a.
- Description
- Rat monoclonal antibody raised against synthetic peptide of Cdkn2a.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Submitted references A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Apicelli AJ, Maggi LB Jr, Hirbe AC, Miceli AP, Olanich ME, Schulte-Winkeler CL, Saporita AJ, Kuchenreuther M, Sanchez J, Weilbaecher K, Weber JD
Molecular and cellular biology 2008 Feb;28(3):1068-80
Molecular and cellular biology 2008 Feb;28(3):1068-80
Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Klochendler-Yeivin A, Picarsky E, Yaniv M
Molecular and cellular biology 2006 Apr;26(7):2661-74
Molecular and cellular biology 2006 Apr;26(7):2661-74
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF
Hybridoma and hybridomics 2004 Oct;23(5):293-300
Hybridoma and hybridomics 2004 Oct;23(5):293-300
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF
Hybridoma and hybridomics 2004 Oct;23(5):293-300
Hybridoma and hybridomics 2004 Oct;23(5):293-300
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2agene (lane 1) and from wild type MEF.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent detection of Cdkn2a in wild type MEF at passage 5 (images 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (images 3, 4) using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cdkn2a monoclonal antibody, clone 12-A1-1 using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a.
- Validation comment
- Immunoprecipitation
