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LearnMAB2417
antibody from Abnova Corporation
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- MAB2417 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2417, RRID:AB_10632522
- Product name
- Cdkn2a monoclonal antibody, clone 5-C3-1
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide corresponding to amino acids 54-75 of mouse Cdkn2a.
- Description
- Rat monoclonal antibody raised against synthetic peptide of Cdkn2a.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Submitted references Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Klochendler-Yeivin A, Picarsky E, Yaniv M
Molecular and cellular biology 2006 Apr;26(7):2661-74
Molecular and cellular biology 2006 Apr;26(7):2661-74
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF
Hybridoma and hybridomics 2004 Oct;23(5):293-300
Hybridoma and hybridomics 2004 Oct;23(5):293-300
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF
Hybridoma and hybridomics 2004 Oct;23(5):293-300
Hybridoma and hybridomics 2004 Oct;23(5):293-300
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2a gene (lane 1) and from wild type MEF.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent detection of Cdkn2a using Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417). Wild type MEF at passage 5 (panels 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (panels 3, 4).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cdkn2a using Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a.
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Cdkn2a on sections from a wild type mouse testis taken at P11 were probed with Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417) (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue).
- Validation comment
- Immunohistochemistry
