Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-0078-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD7 Monoclonal Antibody (4H9), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation. CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma. Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1. Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 4H9
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Casein kinase 2 controls the survival of normal thymic and leukemic γδ T cells via promotion of AKT signaling.
Trained immunity in newborn infants of HBV-infected mothers.
Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
Overexpression of CD7 in classical Hodgkin lymphoma-infiltrating T lymphocytes.
Morphologic and immunologic characterization of 50 peripheral T-cell lymphomas.
A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies.
Ribeiro ST, Tesio M, Ribot JC, Macintyre E, Barata JT, Silva-Santos B
Leukemia 2017 Jul;31(7):1603-1610
Leukemia 2017 Jul;31(7):1603-1610
Trained immunity in newborn infants of HBV-infected mothers.
Hong M, Sandalova E, Low D, Gehring AJ, Fieni S, Amadei B, Urbani S, Chong YS, Guccione E, Bertoletti A
Nature communications 2015 Mar 25;6:6588
Nature communications 2015 Mar 25;6:6588
Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
Jo J, Tan AT, Ussher JE, Sandalova E, Tang XZ, Tan-Garcia A, To N, Hong M, Chia A, Gill US, Kennedy PT, Tan KC, Lee KH, De Libero G, Gehring AJ, Willberg CB, Klenerman P, Bertoletti A
PLoS pathogens 2014 Jun;10(6):e1004210
PLoS pathogens 2014 Jun;10(6):e1004210
IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
Tang XZ, Jo J, Tan AT, Sandalova E, Chia A, Tan KC, Lee KH, Gehring AJ, De Libero G, Bertoletti A
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3142-52
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3142-52
Overexpression of CD7 in classical Hodgkin lymphoma-infiltrating T lymphocytes.
Seegmiller AC, Karandikar NJ, Kroft SH, McKenna RW, Xu Y
Cytometry. Part B, Clinical cytometry 2009 May;76(3):169-74
Cytometry. Part B, Clinical cytometry 2009 May;76(3):169-74
Morphologic and immunologic characterization of 50 peripheral T-cell lymphomas.
Weiss LM, Crabtree GS, Rouse RV, Warnke RA
The American journal of pathology 1985 Feb;118(2):316-24
The American journal of pathology 1985 Feb;118(2):316-24
A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies.
Link M, Warnke R, Finlay J, Amylon M, Miller R, Dilley J, Levy R
Blood 1983 Oct;62(4):722-8
Blood 1983 Oct;62(4):722-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0048-42) and Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD7 FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye