697801

antibody from BioLegend

Targeting: CLDN1 ILVASC, SEMP1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

697801

Provider

BioLegend

Proper citation

BioLegend Cat#697801, RRID:AB_2687205

Product name

Purified anti-Claudin-1

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

9E12C72

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from 293T (negative control), A431 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Claudin-1 (clone 9E12C72) antibody, competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Claudin-1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: molecular weight ladder and M* indicates extended exposure of the molecular weight ladder.

Conjugate

Unconjugated