RQ6328

antibody from NSJ Bioreagents

Targeting: MMP15 MT2-MMP, MTMMP2, SMCP-2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6328

Provider

NSJ Bioreagents

Product name

MT2-MMP Antibody / MMP15

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the MT2-MMP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human A431, 2) human HEK293, 3) human U-2 OS, 4) rat RH-35, 5) mouse liver and 6) mouse testis lysate with MT2-MMP antibody. Predicted molecular weight ~74 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HepG2 cells with MT2-MMP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Caco-2 cells with MT2-MMP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MT2-MMP antibody.