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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Western blot [6]
- Immunohistochemistry [1]
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- Product number
- NBP1-76963 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76963, RRID:AB_11017686
- Product name
- Rabbit Polyclonal Bim Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). NBP1-76963 can detect all three isoforms.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RIPK1 regulates survival of human melanoma cells upon endoplasmic reticulum stress through autophagy.
Apoptosis induced by Ginkgo biloba (EGb761) in melanoma cells is Mcl-1-dependent.
OBATOCLAX and ABT-737 induce ER stress responses in human melanoma cells that limit induction of apoptosis.
Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells.
Enhanced T cell apoptosis within Drak2-deficient mice promotes resistance to autoimmunity.
Thymidine-phosphorothioate oligonucleotides induce activation and apoptosis of CLL cells independently of CpG motifs or BCL-2 gene interference.
The histone deacetylase inhibitor suberic bishydroxamate regulates the expression of multiple apoptotic mediators and induces mitochondria-dependent apoptosis of melanoma cells.
Luan Q, Jin L, Jiang CC, Tay KH, Lai F, Liu XY, Liu YL, Guo ST, Li CY, Yan XG, Tseng HY, Zhang XD
Autophagy 2015;11(7):975-94
Autophagy 2015;11(7):975-94
Apoptosis induced by Ginkgo biloba (EGb761) in melanoma cells is Mcl-1-dependent.
Wang Y, Lv J, Cheng Y, Du J, Chen D, Li C, Zhang J
PloS one 2015;10(4):e0124812
PloS one 2015;10(4):e0124812
OBATOCLAX and ABT-737 induce ER stress responses in human melanoma cells that limit induction of apoptosis.
Wroblewski D, Jiang CC, Croft A, Farrelly ML, Zhang XD, Hersey P
PloS one 2013;8(12):e84073
PloS one 2013;8(12):e84073
Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells.
Zhang LJ, Hao YZ, Hu CS, Ye Y, Xie QP, Thorne RF, Hersey P, Zhang XD
Anti-cancer drugs 2008 Feb;19(2):159-66
Anti-cancer drugs 2008 Feb;19(2):159-66
Enhanced T cell apoptosis within Drak2-deficient mice promotes resistance to autoimmunity.
Ramos SJ, Hernandez JB, Gatzka M, Walsh CM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):7606-16
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):7606-16
Thymidine-phosphorothioate oligonucleotides induce activation and apoptosis of CLL cells independently of CpG motifs or BCL-2 gene interference.
Castro JE, Prada CE, Aguillon RA, Kitada S, Fukuda T, Motta M, Wu C, Dicker F, Sun G, Wang JY, Carson DA, Reed JC, Kipps TJ
Leukemia 2006 Apr;20(4):680-8
Leukemia 2006 Apr;20(4):680-8
The histone deacetylase inhibitor suberic bishydroxamate regulates the expression of multiple apoptotic mediators and induces mitochondria-dependent apoptosis of melanoma cells.
Zhang XD, Gillespie SK, Borrow JM, Hersey P
Molecular cancer therapeutics 2004 Apr;3(4):425-35
Molecular cancer therapeutics 2004 Apr;3(4):425-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-76963] - Analysis of Bim in K562 (K) and A549 (A) whole cell lysates with Bim antibody at 1 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line: Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane. Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell Lines Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Tissue Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: Human thymus Lane 2: Human colon
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.
