35-6300

antibody from Invitrogen Antibodies

Targeting: MAGEA1 CT1.1, MAGE1, MGC9326

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

35-6300

Provider

Invitrogen Antibodies

Product name

MAGE1 Monoclonal Antibody (6C1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

6C1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

A Preliminary Study of the Cross-Reactivity of Canine MAGE-A with Hominid Monoclonal Antibody 6C1 in Canine Mammary Gland Tumors: An Attractive Target for Cancer Diagnostic, Prognostic and Immunotherapeutic Development in Dogs.

Srisawat W, Nambooppha B, Pringproa K, Thongtharb A, Prachasilchai W, Sthitmatee N
Veterinary sciences 2020 Aug 10;7(3)

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Extramammary Paget disease shows differential expression of B7 family members B7-H3, B7-H4, PD-L1, PD-L2 and cancer/testis antigens NY-ESO-1 and MAGE-A.

Pourmaleki M, Young JH, Socci ND, Chiang S, Edelweiss M, Li Y, Zhang M, Roshal L, Chi DS, Busam KJ, Mellinghoff IK, Hollmann TJ
Oncotarget 2019 Oct 22;10(58):6152-6167

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Immunomodulatory action of SGI-110, a hypomethylating agent, in acute myeloid leukemia cells and xenografts.

Srivastava P, Paluch BE, Matsuzaki J, James SR, Collamat-Lai G, Karbach J, Nemeth MJ, Taverna P, Karpf AR, Griffiths EA
Leukemia research 2014 Nov;38(11):1332-41

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Melanoma-associated cancer-testis antigen 16 (CT16) regulates the expression of apoptotic and antiapoptotic genes and promotes cell survival.

Nylund C, Rappu P, Pakula E, Heino A, Laato L, Elo LL, Vihinen P, Pyrhönen S, Owen GR, Larjava H, Kallajoki M, Heino J
PloS one 2012;7(9):e45382

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Heterogeneous expression of GAGE, NY-ESO-1, MAGE-A and SSX proteins in esophageal cancer: Implications for immunotherapy.

Akcakanat A, Kanda T, Tanabe T, Komukai S, Yajima K, Nakagawa S, Ohashi M, Hatakeyama K
International journal of cancer 2006 Jan 1;118(1):123-8

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Expression of family A melanoma antigen in human gastric carcinoma.

Jung EJ, Kim MA, Lee HS, Yang HK, Lee YM, Lee BL, Kim WH
Anticancer research 2005 May-Jun;25(3B):2105-11

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Positive cross-reactivity of MAb 6C1, testis and canines. The detection of MAGE antigen in canine testicular tissue sections at different dilutions using IHC method (40x). ( a - e ) MAb 6C1 in phosphate buffer solution (PBS) in dilutions of 1:100, 1:200, 1:400, 1:800 and 1:1000, respectively; ( f ) negative control (normal mouse serum in PBS in a dilution of 1:200).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Positive cross-reactivity of MAb 6C1, mammary gland tumors and canines. Location of MAGE expression in the complex carcinoma type of malignant mammary gland tumor section Ma1: ( a ) HE stained, 40x; ( b ) IHC-stained-positive, 40x; ( c ) IHC-stained-positive, 100x black arrows are representative of nuclear expression pattern; red arrows are representative of nuclear-cytoplasmic expression pattern.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Positive cross-reactivity of MAb 6C1, mammary gland tumors and canines. Location of MAGE expression in the simple tubular carcinoma type of malignant mammary gland tumor section Ma2: ( a ) HE stained, 40x; ( b ) IHC-stained-positive, 40x, ( c ) IHC-stained-positive, 100x, black arrows are representative of nuclear expression pattern.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Representative images of immunohistochemistry for B7-H3, B7-H4, PD-L1, PD-L2, MAGE-A, NY-ESO-1, MHC-I, and B2M in in situ and invasive EMPD. IHC images for each marker along with the corresponding H&E image for each case are shown for in situ and invasive EMPD.