643804

antibody from BioLegend

Targeting: ACTB

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

643804

Provider

BioLegend

Proper citation

BioLegend Cat#643804, RRID:AB_2565573

Product name

Alexa Fluor® 594 anti-β-actin

Antibody type

Monoclonal

Antigen

Other

Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

2F1-1

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

MCF7 mammary gland cells were fixed with cold methanol for ten minutes and blocked with 5% FBS for 30 minutes. Then, the cells were stained with 2.5 µg/mL of anti β-actin (clone 2F1-1) Alexa Fluor® 594 (red) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye