Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F40216 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- b-Actin Antibody
- Antibody type
- Monoclonal
- Antigen
- This b-Actin antibody was produced from a mouse immunized with ACTB recombinant protein.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 137CT26.1.1
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the b-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of b-Actin antibody in 1) K562, 2) HL-60, 3) HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of b-Actin antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human spleen using b-Actin antibody at 1:25 dilution.
