Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- MA1-140-A647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Actin Monoclonal Antibody (15G5A11/E2), Alexa Fluor™ 647
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- 15G5A11/E2
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Green Synthesized Honokiol Transfersomes Relieve the Immunosuppressive and Stem-Like Cell Characteristics of the Aggressive B16F10 Melanoma.
Ezzeldeen Y, Swidan S, ElMeshad A, Sebak A
International journal of nanomedicine 2021;16:5693-5712
International journal of nanomedicine 2021;16:5693-5712
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of beta-Actin was performed by loading various cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and blocked with Sea Block blocking buffer for at least 1 hour. The membrane was probed with a AlexaFluor647-conjugated beta-Actin monoclonal antibody (Product # MA1-140-A647) at a dilution of 1:500 for over night at 4C on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging system.
- Conjugate
- Red dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 10 Role of HKTs on the immunosuppressive (TGFbeta and CD47) and stem-like cell (CD133) markers of TAM-activated ( A ) and TAF-activated ( B ) B16F10 melanoma cells. TGF-beta concentration was analyzed with ELISA, while CD47 and CD133 were analyzed with WB. Bands for CD47, CD133, and beta-actin proteins were visualized by enhanced chemiluminescence (ECL Plus) and quantified relative to beta-actin using densitometry and Molecular Analyst Software. Uncropped blots of CD47, CD133, and beta-actin proteins are provided in the supplementary materials (Figure S1A-C, respectively). * p< 0.05; ***** p< 0.001; ** p< 0.0001.
- Conjugate
- Red dye