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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- V9727 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Actin Beta Antibody / ACTB
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antibody clone number
- ACTB/1109
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Aliquot the Actin Beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of human Raji cell lysate using Actin Beta antibody (clone ACTB/1109). Predicted molecular weight ~42 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with Actin Beta antibody (clone ACTB/1109). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
