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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- ARG51837 - Provider product page
- Provider
- Arigo
- Product name
- anti-Cdc25B phospho (Ser149) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) stained with anti-Cdc25B (phospho Ser149) antibody ARG51837.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), stained with anti-Cdc25B (phospho Ser149) antibody ARG51837.
