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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB18322 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18322, RRID:AB_10716830
- Product name
- CDC25B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDC25B.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1-Cdk1 at the centrosome.
Binding of 14-3-3beta but not 14-3-3sigma controls the cytoplasmic localization of CDC25B: binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B.
Lindqvist A, Källström H, Lundgren A, Barsoum E, Rosenthal CK
The Journal of cell biology 2005 Oct 10;171(1):35-45
The Journal of cell biology 2005 Oct 10;171(1):35-45
Binding of 14-3-3beta but not 14-3-3sigma controls the cytoplasmic localization of CDC25B: binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B.
Uchida S, Kuma A, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Ishizaka Y, Yamashita K
Journal of cell science 2004 Jun 15;117(Pt 14):3011-20
Journal of cell science 2004 Jun 15;117(Pt 14):3011-20
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Raw 264.7 cells, treated with UV (15 mins), using CDC25B polyclonal antibody (Cat # PAB18322).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells, using CDC25B polyclonal antibody (Cat # PAB18322).Peptide "+" means "peptide blocking".
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissue using CDC25B polyclonal antibody (Cat # PAB18322).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
