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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 10644-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDC25B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: NDACLSIVH SLMCHRQGGE SETFAKRAIE SLVKKLKEKK DELDSLITAI TTNGAHPSKC VTIQRTLDGR LQVAGRKGFP HVIYARLWRW PDLHKNELKH VKYCQYAFDL KCDSVCVNPY HYERVVSPGI DLSGLTLQSN APSSMMVKDE YVHDFEGQPS LSTEGHSIQT IQHPPSNRAS TETYSTPALL APSESNATST ANFPNIPVAS TSQPAS (13-227 aa encoded by BC002379)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma using 10644-1-AP (CDC25B antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CDC25B (IP:10644-1-AP, 4ug; Detection:10644-1-AP 1:300) with mouse liver tissue lysate 4000ug.
