RQ7156

antibody from NSJ Bioreagents

Targeting: CDC25B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7156

Provider

NSJ Bioreagents

Product name

CDC25B Antibody / M-phase inducer phosphatase 2

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the M-phase inducer phosphatase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat liver, 2) rat brain, 3) rat kidney, 4) rat testis, 5) mouse liver, 6) mouse brain, 7) mouse kidney and 8) mouse testis tissue lysate with M-phase inducer phosphatase 2 antibody. Expected molecular weight: 61~67 kDa (isoforms 1-4).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) 293T, 2) Caco-2, 3) MOLT4, 4) Raji, 5) HeLa, 6) HaCaT and 7) SiHa cell lysate with M-phase inducer phosphatase 2 antibody. Expected molecular weight: 61~67 kDa (isoforms 1-4).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-87 MG cells with M-phase inducer phosphatase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= M-phase inducer phosphatase 2 antibody.