Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-05035 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05035, RRID:AB_1520617
- Product name
- Goat Polyclonal Clathrin light chain + heavy chain Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize all reported isoforms of light chains a and b.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
internal region
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.
Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization.
Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS
Proceedings of the National Academy of Sciences of the United States of America 2008 Jan 8;105(1):168-73
Proceedings of the National Academy of Sciences of the United States of America 2008 Jan 8;105(1):168-73
Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization.
Okamoto CT, Duman JG, Tyagarajan K, McDonald KL, Jeng YY, McKinney J, Forte TM, Forte JG
American journal of physiology. Cell physiology 2000 Sep;279(3):C833-51
American journal of physiology. Cell physiology 2000 Sep;279(3):C833-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.