10852-1-AP

antibody from Invitrogen Antibodies

Targeting: CLTA Lca

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10852-1-AP

Provider

Invitrogen Antibodies

Product name

CLTA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAELDPFGA PAGAPGGPAL GNGVAGAGEE DPAAAFLAQQ ESEIAGIEND EAFAILDGGA PGPQPHGEPP GGPDAVDGVM NGEYYQESNG PTDSYAAISQ VDRLQSEPES IRKWREEQME RLEALDANSR KQEAEWKEKA IKELEEWYAR QDEQLQKTKA NNRAAEEAFV NDIDESSPGT EWERVARLCD FNPKSSKQAK DVSRMRSVLI SLKQAPLVH (1-218 aa encoded by BC019287 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.47 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HEK-293 cells using 10852-1-AP (CLTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 10852-1-AP) (CLTA antibody) at dilution of 1:500 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 10852-1-AP (CLTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10852-1-AP ( CLTA Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10852-1-AP ( CLTA Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CLTA (IP:10852-1-AP, 4ug; Detection:10852-1-AP 1:800) with mouse brain tissue lysate 4000ug.