Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-15190-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD43/Sialophorin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD43/Sialophorin Antibody (DF-T1) [NBP2-15190] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Antibody (DF-T1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (DF-T1) [NBP2-15190] - Formalin-paraffin human spleen stained with CD43 Ab (DF-T1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD43/Sialophorin Antibody (DF-T1) [NBP2-15190] - CD43 expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD43 antibody (0.5ug/10^6 cells) or isotype control this antibody following a surface staining protocol. PE-conjugated goat anti-mouse IgG secondary antibody this antibody used to identify the positive staining on PPI negative lymphocyte gated population.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD43/Sialophorin Antibody (DF-T1) [NBP2-15190] - Flow Cytometric Analysis of PFA-fixed K562 cells using CD43/Sialophorin Antibody (DF-T1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).