Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- 10191-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DFF45/DFFA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GVPESGEIR TLKPCLLRRN YSREQHGVAA SCLEDLRSKA CDILAIDKSL TPVTLVLAED GTIVDDDDYF LCLPSNTKFV ALASNEKWAY NNSDGGTAWI SQESFDVDET DSGAGLKWKN VARQLKEDLS SIILLSEEDL QMLVDAPCSD LAQELRQSCA TVQRLQHTLQ QVLDQREEVR QSKQLLQLYL QALEKEGSLL SKQEESKAAF GEEVDAVDTG ISRETSSDVA LASHILTALR EKQAPELSLS SQDLELVTKE DPKALAVALN WDIKKTETVQ EACERELALR LQQTQSLHSL RSISASKASP PGDLQNPKRA RQ (8-328 aa encoded by BC000037)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HEK-293 cells using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DFF45/DFFA (IP:10191-2-AP, 3ug; Detection:10191-2-AP 1:1000) with HEK-293 cells lysate 2500ug.