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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX100281 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100281, RRID:AB_1240877
- Product name
- GAD65 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX100281 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (10 ug of whole cell lysate) A: rat brain 7.5% SDS PAGE GTX100281 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:20000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GAD65 antibody [C2C3], C-term detects GAD65 protein expression by immunofluorescent analysis.Sample: Cultured Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920)..
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody [C2C3], C-term (GTX100281) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).
