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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA308367 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal antibody to GAD65 (glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa))
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody to GAD65 (glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa))
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GAD2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Fine Mapping of Glutamate Decarboxylase 65 Epitopes Reveals Dependency on Hydrophobic Amino Acids for Specific Interactions.
Valdarnini N, Holm B, Hansen P, Rovero P, Houen G, Trier N
International journal of molecular sciences 2019 Jun 14;20(12)
International journal of molecular sciences 2019 Jun 14;20(12)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: mouse brain. 7.5% SDS PAGE. TA308367 diluted at 1:5000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: rat brain. 7.5% SDS PAGE. TA308367 diluted at 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAD65(TA308367) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2500.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody (TA308367) dilution: 1:500.
- Validation comment
- IHC
