Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA324381 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal GAD2 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal GAD2 Antibody (Center)
- Reactivity
- Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GAD2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.47 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD2 Antibody (Center) (Cat. #TA324381) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody (Center)(Cat. #TA324381). TA324381 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAD2 Antibody (Center)(Cat. #TA324381) with 293 cell followed by Alexa Fluor?? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD2 Antibody (Center) (Cat. #TA324381)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC