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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP3013a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3013a, RRID:AB_637568
- Product name
- Phospho-ABL1(Y245) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- RB7349
- Vial size
- 400 µl
- Storage
- Store at 2 to 8°C.Antibody is stable for 52 months.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ABL Pab (Cat. #AP3013a) in mouse colon tissue lysate (35ug/lane). ABL(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Phospho-ABL1-Y245 Antibody(Cat#AP3013a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
