MA5-14395

antibody from Invitrogen Antibodies

Targeting: ABL1 ABL, c-ABL, JTK7, p150

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14395

Provider

Invitrogen Antibodies

Product name

Anti-c-Abl Monoclonal Antibody (8E9), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14398 targets c-Abl in immunofluorescence, immunoprecipitation, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with Human. The MA5-14398 immunogen is recombinant Abl protein.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

8E9

Vial size

500 µL

Concentration

0.2 mg/ml

Storage

4° C

References

Submitted references

Phase II trial of imatinib mesylate in patients with recurrent platinum- and taxane-resistant low-grade serous carcinoma of the ovary, peritoneum, or fallopian tube.

Noguera IR, Sun CC, Broaddus RR, Branham D, Levenback CF, Ramirez PT, Sood AK, Coleman RL, Gershenson DM
Gynecologic oncology 2012 Jun;125(3):640-5

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Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation.

Baker MA, Hetherington L, Curry B, Aitken RJ
Developmental biology 2009 Sep 1;333(1):57-66

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Phase II trial of imatinib mesylate in patients with recurrent platinum- and taxane-resistant epithelial ovarian and primary peritoneal cancers.

Coleman RL, Broaddus RR, Bodurka DC, Wolf JK, Burke TW, Kavanagh JJ, Levenback CF, Gershenson DM
Gynecologic oncology 2006 Apr;101(1):126-31

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Expression of imatinib mesylate-targeted kinases in endometrial carcinoma.

Slomovitz BM, Broaddus RR, Schmandt R, Wu W, Oh JC, Ramondetta LM, Burke TW, Gershenson DM, Lu KH
Gynecologic oncology 2004 Oct;95(1):32-6

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Expression of c-ABL, c-KIT, and platelet-derived growth factor receptor-beta in ovarian serous carcinoma and normal ovarian surface epithelium.

Schmandt RE, Broaddus R, Lu KH, Shvartsman H, Thornton A, Malpica A, Sun C, Bodurka DC, Gershenson DM
Cancer 2003 Aug 15;98(4):758-64

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of c-Abl (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody (Product # MA5-14398) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Conjugate

Biotin

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human placenta stained with cbl antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.

Conjugate

Biotin