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- Product number
- GTX100053 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100053, RRID:AB_1949674
- Product name
- Apolipoprotein E antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Increased expression of ApoE and protection from amyloid-beta toxicity in transmitochondrial cybrids with haplogroup K mtDNA.
Thaker K, Chwa M, Atilano SR, Coskun P, Cáceres-Del-Carpio J, Udar N, Boyer DS, Jazwinski SM, Miceli MV, Nesburn AB, Kuppermann BD, Kenney MC
Neurobiology of disease 2016 Sep;93:64-77
Neurobiology of disease 2016 Sep;93:64-77
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (2.5 ?g of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE GTX100053 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.A. 30 ?g HepG2 whole cell lysate/extract12% SDS-PAGEApolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human tissue extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human plasma (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human plasma (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.Blue: Hoechst 33342 staining.
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- GeneTex (provider)
- Main image
- Experimental details
- Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- Main image
- Experimental details
- Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.IP Sample: HepG2 whole cell lysate/extractA : 30 £gg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cellsB : Control with 3 £gg of pre-immune rabbit IgGC : Immunoprecipitation of Apolipoprotein E by 3 £gg of Apolipoprotein E antibody [C2C3], C-term (GTX100053) 10% SDS-PAGE The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Apolipoprotein E antibody [C2C3], C-term detects APOE protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:500.
