600-101-197

antibody from Rockland Immunochemicals, Inc.

Targeting: APOE AD2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-101-197

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-101-197, RRID:AB_2227401

Product name

Anti-APOLIPOPROTEIN E (GOAT) Antibody - 600-101-197

Antibody type

Polyclonal

Vial size

500 µl

References

Submitted references

Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine β-synthase-deficient rabbit generated using CRISPR/Cas9.

Zhang T, Lu R, Chen Y, Yuan Y, Song S, Yan K, Zha Y, Zhuang W, Cheng Y, Liang J
Lipids in health and disease 2020 Oct 14;19(1):224

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Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.

Lu R, Yuan T, Wang Y, Zhang T, Yuan Y, Wu D, Zhou M, He Z, Lu Y, Chen Y, Fan J, Liang J, Cheng Y
EBioMedicine 2018 Oct;36:29-38

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Quantification of the host response proteome after herpes simplex virus type 1 infection.

Berard AR, Coombs KM, Severini A
Journal of proteome research 2015 May 1;14(5):2121-42

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Comparative proteomic analyses of two reovirus T3D subtypes and comparison to T1L identifies multiple novel proteins in key cellular pathogenic pathways.

Berard AR, Severini A, Coombs KM
Proteomics 2015 Jun;15(12):2113-35

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit Anti Apolipoprotein E Antibody. Lane 1: rat glial whole cell lysate. Load: 40 µg per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4°C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): ~55kDa.

Validation comment

Western Blot