Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- AM02001PU-N - Provider product page
- Provider
- OriGene
- Product name
- CEACAM6 mouse monoclonal antibody, clone 9A6, Purified
- Antibody type
- Monoclonal
- Description
- CEACAM6 mouse monoclonal antibody, clone 9A6, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM6
- Isotype
- IgG
- Antibody clone number
- 9A6
- Vial size
- 100 µg
- Concentration
- 1.8 mg/ml
Submitted references Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens.
Schmitter T, Agerer F, Peterson L, Munzner P, Hauck CR
The Journal of experimental medicine 2004 Jan 5;199(1):35-46
The Journal of experimental medicine 2004 Jan 5;199(1):35-46
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2: FACS analysis of BOSC23 cells using CEACAM6 antibody Clone 9A6. BOSC23 cells were transiently transfected with an expressionvector encoding either CEACAM6 (red curve) or an irrelevantprotein (control transfectant). Binding of 9A6 was detected with a PE conjugated secondary antibody. A positive signal wasobtained only with CEACAM6 transfected cells.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 1: Specificity testing of 9A6. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-9A6 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM6-expressing cells (red curves).
- Validation comment
- FC