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- Antigen structure
- References [11]
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- Flow cytometry [2]
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- Product number
- AM02001PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM02001PU-N, RRID:AB_977069
- Product name
- anti CD66c / CEACAM6
- Antibody type
- Monoclonal
- Antigen
- Tumor cell lines expressing CEACAM6 (1,2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9A6
- Vial size
- 0.1 mg
- Concentration
- 1.8 mg/ml
Submitted references Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion.
Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.
Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM.
The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays.
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.
Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice.
Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens.
Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens.
Rolhion N, Barnich N, Bringer MA, Glasser AL, Ranc J, Hébuterne X, Hofman P, Darfeuille-Michaud A
Gut 2010 Oct;59(10):1355-62
Gut 2010 Oct;59(10):1355-62
Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.
Singer BB, Scheffrahn I, Kammerer R, Suttorp N, Ergun S, Slevogt H
PloS one 2010 Jan 18;5(1):e8747
PloS one 2010 Jan 18;5(1):e8747
Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
Kanderová V, Hrusák O, Kalina T
Experimental hematology 2010 Aug;38(8):653-660.e1
Experimental hematology 2010 Aug;38(8):653-660.e1
Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM.
Carvalho FA, Barnich N, Sivignon A, Darcha C, Chan CH, Stanners CP, Darfeuille-Michaud A
The Journal of experimental medicine 2009 Sep 28;206(10):2179-89
The Journal of experimental medicine 2009 Sep 28;206(10):2179-89
The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays.
Yue T, Goldstein IJ, Hollingsworth MA, Kaul K, Brand RE, Haab BB
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1697-707
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1697-707
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Sémiramoth N, Gleizes A, Turbica I, Sandré C, Gorges R, Kansau I, Servin A, Chollet-Martin S
Journal of leukocyte biology 2009 Feb;85(2):310-21
Journal of leukocyte biology 2009 Feb;85(2):310-21
Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice.
Chan CH, Camacho-Leal P, Stanners CP
PloS one 2007 Dec 26;2(12):e1353
PloS one 2007 Dec 26;2(12):e1353
Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
Blood 2007 Dec 15;110(13):4223-33
Blood 2007 Dec 15;110(13):4223-33
Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens.
Kalina T, Vaskova M, Mejstrikova E, Madzo J, Trka J, Stary J, Hrusak O
BMC cancer 2005 Apr 12;5:38
BMC cancer 2005 Apr 12;5:38
Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens.
Schmitter T, Agerer F, Peterson L, Munzner P, Hauck CR
The Journal of experimental medicine 2004 Jan 5;199(1):35-46
The Journal of experimental medicine 2004 Jan 5;199(1):35-46
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1: Specificity testing of 9A6. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-9A6 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM6-expressing cells (red curves).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2: FACS analysis of BOSC23 cells using AM02001PU-N CEACAM6 antibody Clone 9A6. BOSC23 cells were transiently transfected with an expressionvector encoding either CEACAM6 (red curve) or an irrelevantprotein (control transfectant). Binding of 9A6 was detected with a PE conjugated secondary antibody. A positive signal wasobtained only with CEACAM6 transfected cells.
