Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 692802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#692802, RRID:AB_2632769
- Product name
- Purified anti-RUNX2
- Antibody type
- Monoclonal
- Description
- Runt Related Transcription Factor 2 (RUNX2), also known as CBFA1, belongs to the mammalian Runt-related transcription factor (RUNX) family. The RUNX family is comprised of three members: RUNX1, RUNX2, and RUNX3. The RUNX proteins are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. Among these members, RUNX2 contains one Runt domain and specifically expresses in osteoblasts. It plays critical roles in skeletal mineralization, osteoblast differentiation of mesenchymal stem cells, chondrocyte hypertrophy, endothelial cell migration, and vascular invasion of developing bones. Defects in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) and defective endochondral and intramembranous bone formation. In some cases, defects in RUNX2 are exclusively associated with dental anomalies. RUNX2 binds DNA as a monomer or as a subunit of a heterodimeric complex. In osteoblasts, RUNX2 supports transcriptional activation by synergizing with SPEN/MINT. RUNX2 also functions to inhibit KAT6B (MYST4)-dependent transcriptional activation.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W15212A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate (15 µg/lane) from HOS cells, NIH3T3 cells, and HeLa cells were resolved by 4-12% Bis-tris electrophoresis, transferred to nitrocellulose, and probed with 0.005 µg/mL purified anti-RUNX2 (clone W15212A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
- Conjugate
- Unconjugated