Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2006 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RUNX2/CBFA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RUNX2/CBFA1.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q13950
- Isotype
- IgG
- Antibody clone number
- 232902
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate Microparticles.
Collagen type XV and the 'osteogenic status'.
Cell-matrix signals specify bone endothelial cells during developmental osteogenesis.
The Selective Serotonin Reuptake Inhibitor Fluoxetine Directly Inhibits Osteoblast Differentiation and Mineralization During Fracture Healing in Mice.
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
GATA4 is essential for bone mineralization via ERα and TGFβ/BMP pathways.
Identification of a clonally expanding haematopoietic compartment in bone marrow.
Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease.
Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
Emergence of patterned stem cell differentiation within multicellular structures.
Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate.
Day AGE, Francis WR, Fu K, Pieper IL, Guy O, Xia Z
Stem cells international 2018;2018:4258613
Stem cells international 2018;2018:4258613
Collagen type XV and the 'osteogenic status'.
Lisignoli G, Lambertini E, Manferdini C, Gabusi E, Penolazzi L, Paolella F, Angelozzi M, Casagranda V, Piva R
Journal of cellular and molecular medicine 2017 Sep;21(9):2236-2244
Journal of cellular and molecular medicine 2017 Sep;21(9):2236-2244
Cell-matrix signals specify bone endothelial cells during developmental osteogenesis.
Langen UH, Pitulescu ME, Kim JM, Enriquez-Gasca R, Sivaraj KK, Kusumbe AP, Singh A, Di Russo J, Bixel MG, Zhou B, Sorokin L, Vaquerizas JM, Adams RH
Nature cell biology 2017 Mar;19(3):189-201
Nature cell biology 2017 Mar;19(3):189-201
The Selective Serotonin Reuptake Inhibitor Fluoxetine Directly Inhibits Osteoblast Differentiation and Mineralization During Fracture Healing in Mice.
Bradaschia-Correa V, Josephson AM, Mehta D, Mizrahi M, Neibart SS, Liu C, Kennedy OD, Castillo AB, Egol KA, Leucht P
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Apr;32(4):821-833
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Apr;32(4):821-833
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Ramasamy SK, Kusumbe AP, Wang L, Adams RH
Nature 2014 Mar 20;507(7492):376-380
Nature 2014 Mar 20;507(7492):376-380
Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
Kusumbe AP, Ramasamy SK, Adams RH
Nature 2014 Mar 20;507(7492):323-328
Nature 2014 Mar 20;507(7492):323-328
GATA4 is essential for bone mineralization via ERα and TGFβ/BMP pathways.
Güemes M, Garcia AJ, Rigueur D, Runke S, Wang W, Zhao G, Mayorga VH, Atti E, Tetradis S, Péault B, Lyons K, Miranda-Carboni GA, Krum SA
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2014 Dec;29(12):2676-87
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2014 Dec;29(12):2676-87
Identification of a clonally expanding haematopoietic compartment in bone marrow.
Wang L, Benedito R, Bixel MG, Zeuschner D, Stehling M, Sävendahl L, Haigh JJ, Snippert H, Clevers H, Breier G, Kiefer F, Adams RH
The EMBO journal 2013 Jan 23;32(2):219-30
The EMBO journal 2013 Jan 23;32(2):219-30
Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
Pai A, Leaf EM, El-Abbadi M, Giachelli CM
The American journal of pathology 2011 Feb;178(2):764-73
The American journal of pathology 2011 Feb;178(2):764-73
Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease.
Todoerti K, Lisignoli G, Storti P, Agnelli L, Novara F, Manferdini C, Codeluppi K, Colla S, Crugnola M, Abeltino M, Bolzoni M, Sgobba V, Facchini A, Lambertenghi-Deliliers G, Zuffardi O, Rizzoli V, Neri A, Giuliani N
Experimental hematology 2010 Feb;38(2):141-53
Experimental hematology 2010 Feb;38(2):141-53
Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
Kidney international 2009 Jun;75(12):1297-1307
Kidney international 2009 Jun;75(12):1297-1307
Emergence of patterned stem cell differentiation within multicellular structures.
Ruiz SA, Chen CS
Stem cells (Dayton, Ohio) 2008 Nov;26(11):2921-7
Stem cells (Dayton, Ohio) 2008 Nov;26(11):2921-7
Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF, Freedman LP, Brown M
The EMBO journal 2008 Feb 6;27(3):535-45
The EMBO journal 2008 Feb 6;27(3):535-45
The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate.
Curran JM, Chen R, Hunt JA
Biomaterials 2006 Sep;27(27):4783-93
Biomaterials 2006 Sep;27(27):4783-93
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RUNX2/CBFA1 in U2OS Human Cell Line. RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.