Explore
Validate
LearnH00000860-M06
antibody from Novus Biologicals
Targeting: RUNX2
AML3, CBFA1, CCD, CCD1, PEBP2A1, PEBP2aA1
Western blot ELISA Immunocytochemistry Immunohistochemistry
Functional assay Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000860-M06 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000860-M06, RRID:AB_828679
- Product name
- Mouse Monoclonal RUNX2/CBFA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RUNX2 (3F5)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.
Granulation Tissue Eroding the Subchondral Bone Also Promotes New Bone Formation in Ankylosing Spondylitis.
Thyroid Hormone Receptor-β (TRβ) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.
Osteogenic, stem cell and molecular characterisation of the human induced membrane from extremity bone defects.
Aggregatibacter actinomycetemcomitans lipopolysaccharide regulates bone sialoprotein gene transcription.
Transcription factor Runx2 is a regulator of epithelial-mesenchymal transition and invasion in thyroid carcinomas.
cAMP and fibroblast growth factor 2 regulate bone sialoprotein gene expression in human prostate cancer cells.
Effects of inorganic polyphosphate on bone sialoprotein gene expression.
Sonomoto K, Yamaoka K, Kaneko H, Yamagata K, Sakata K, Zhang X, Kondo M, Zenke Y, Sabanai K, Nakayamada S, Sakai A, Tanaka Y
PloS one 2016;11(4):e0153231
PloS one 2016;11(4):e0153231
Granulation Tissue Eroding the Subchondral Bone Also Promotes New Bone Formation in Ankylosing Spondylitis.
Bleil J, Maier R, Hempfing A, Sieper J, Appel H, Syrbe U
Arthritis & rheumatology (Hoboken, N.J.) 2016 Oct;68(10):2456-65
Arthritis & rheumatology (Hoboken, N.J.) 2016 Oct;68(10):2456-65
Thyroid Hormone Receptor-β (TRβ) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.
Carr FE, Tai PW, Barnum MS, Gillis NE, Evans KG, Taber TH, White JH, Tomczak JA, Jaworski DM, Zaidi SK, Lian JB, Stein JL, Stein GS
Endocrinology 2016 Aug;157(8):3278-92
Endocrinology 2016 Aug;157(8):3278-92
Osteogenic, stem cell and molecular characterisation of the human induced membrane from extremity bone defects.
Gruber HE, Ode G, Hoelscher G, Ingram J, Bethea S, Bosse MJ
Bone & joint research 2016 Apr;5(4):106-15
Bone & joint research 2016 Apr;5(4):106-15
Aggregatibacter actinomycetemcomitans lipopolysaccharide regulates bone sialoprotein gene transcription.
Li X, Zhou L, Takai H, Sasaki Y, Mezawa M, Li Z, Wang Z, Yang L, Wang S, Matsumura H, Kaneko T, Yoshimura A, Ogata Y
Journal of cellular biochemistry 2012 Sep;113(9):2822-34
Journal of cellular biochemistry 2012 Sep;113(9):2822-34
Transcription factor Runx2 is a regulator of epithelial-mesenchymal transition and invasion in thyroid carcinomas.
Niu DF, Kondo T, Nakazawa T, Oishi N, Kawasaki T, Mochizuki K, Yamane T, Katoh R
Laboratory investigation; a journal of technical methods and pathology 2012 Aug;92(8):1181-90
Laboratory investigation; a journal of technical methods and pathology 2012 Aug;92(8):1181-90
cAMP and fibroblast growth factor 2 regulate bone sialoprotein gene expression in human prostate cancer cells.
Li Z, Sasaki Y, Mezawa M, Wang S, Li X, Yang L, Wang Z, Zhou L, Araki S, Matsumura H, Takai H, Ogata Y
Gene 2011 Jan 15;471(1-2):1-12
Gene 2011 Jan 15;471(1-2):1-12
Effects of inorganic polyphosphate on bone sialoprotein gene expression.
Wang Z, Li X, Li Z, Yang L, Sasaki Y, Wang S, Zhou L, Araki S, Mezawa M, Takai H, Ogata Y
Gene 2010 Mar 1;452(2):79-86
Gene 2010 Mar 1;452(2):79-86
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell . [antibody concentration 10 ug/ml]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
