Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AP17861PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP17861PU-N, RRID:AB_10651554
- Product name
- anti ABI1 / SSH3BP1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 87~117 amino acids from the N-terminal region of Human ABI1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ABI1 Antibody (N-term) (AP17861PU-N) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human brain tissue stained with ABI1 Antibody (N-term) Cat.-No AP17861PU-N which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.