NBP2-16703

antibody from Novus Biologicals

Targeting: GNAO1 G-ALPHA-o

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16703

Provider

Novus Biologicals

Product name

Rabbit Polyclonal GO Protein alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GO Protein alpha Antibody [NBP2-16703] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GO Protein alpha Antibody [NBP2-16703] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GO Protein alpha Antibody [NBP2-16703] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: GO Protein alpha Antibody [NBP2-16703] - GNAO1 protein from HeLa whole cell extracts using 5 ug of GNAO1 antibody (NBP2-16703). Western blot analysis was performed using GNAO1 antibody (NBP2-16703). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - Paraffin-embedded colon carcinoma. GNAO1 antibody dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis. Rat pancreas. GNAO1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GO Protein alpha Antibody [NBP2-16703] - GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis. Mouse pancreas. GNAO1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: GO Protein alpha Antibody [NBP2-16703] - Frozen-sectioned mouse cerebellum. Green: GNAO1 stained by GNAO1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.