MAB229

antibody from Novus Biologicals

Targeting: TNFRSF8 CD30, D1S166E, KI-1

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB229

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD30/TNFRSF8 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CD30/TNFRSF8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD30, recombinant human (rh) CD27, and rhCD40 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease.

Tian Y, Seumois G, De-Oliveira-Pinto LM, Mateus J, Herrera-de la Mata S, Kim C, Hinz D, Goonawardhana NDS, de Silva AD, Premawansa S, Premawansa G, Wijewickrama A, Balmaseda A, Grifoni A, Vijayanand P, Harris E, Peters B, Sette A, Weiskopf D
Cell reports 2019 Dec 24;29(13):4482-4495.e4

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Cytoplasmic Pin1 expression is increased in human cutaneous melanoma and predicts poor prognosis.

Chen X, Liu X, Deng B, Martinka M, Zhou Y, Lan X, Cheng Y
Scientific reports 2018 Nov 15;8(1):16867

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Selective redox regulation of cytokine receptor signaling by extracellular thioredoxin-1.

Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, Engelhard J, Winkler M, Dick TP
The EMBO journal 2007 Jul 11;26(13):3086-97

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of CD30/TNFRSF8 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB229, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Other assay

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Human CD30/TNFRSF8 Antibody Enhances IL-6 Secretion in HDLM-2 Cells. Human CD30/TNFRSF8 Antibody Enhances IL-6 Secretion in HDLM-2 Cells.