- GTX108624 - GeneTex CCND1 antibody

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: U87-MG 12% SDS PAGE GTX108624 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Cyclin D1 antibody [N1C3] detects Cyclin D1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Cyclin D1 antibody [N1C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 £gg of Cyclin D1 antibody [N1C3] (GTX108624).Western blot analysis was performed using Cyclin D1 antibody [N1C3] (GTX108624).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108624

Antibody data