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- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- V2006 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Cyclin D1 Antibody
- Antibody type
- Monoclonal
- Antigen
- Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- DCS-6
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Inulanolide A as a new dual inhibitor of NFAT1-MDM2 pathway for breast cancer therapy.
New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress.
Serum vitamin D (25OHD3) levels and the risk of different subtypes of breast cancer: A nested case-control study.
Identification of thyroid tumor cell vulnerabilities through a siRNA-based functional screening.
Estrogen Receptor β Agonists Differentially Affect the Growth of Human Melanoma Cell Lines.
Characterization of a new small bowel adenocarcinoma cell line and screening of anti-cancer drug against small bowel adenocarcinoma.
Expression and inactivation of glycogen synthase kinase 3 alpha/ beta and their association with the expression of cyclin D1 and p53 in oral squamous cell carcinoma progression.
Acetylation-defective mutants of Pparγ are associated with decreased lipid synthesis in breast cancer cells
Vitamin D signaling regulates oral keratinocyte proliferation in vitro and in vivo.
WNT signaling and distant metastasis in colon cancer through transcriptional activity of nuclear β-Catenin depend on active PI3K signaling.
SHIP2 signaling in normal and pathological situations: Its impact on cell proliferation.
Targeting cyclin-dependent kinase 1 (CDK1) but not CDK4/6 or CDK2 is selectively lethal to MYC-dependent human breast cancer cells.
Cyclin D1/cyclin-dependent kinase 4 interacts with filamin A and affects the migration and invasion potential of breast cancer cells.
Adhesion control of cyclin D1 and p27Kip1 levels is deregulated in melanoma cells through BRAF-MEK-ERK signaling.
Epidermal growth factor receptor and cyclin D1 are independently amplified and overexpressed in esophageal squamous cell carcinoma.
Cyclin D1 represses p300 transactivation through a cyclin-dependent kinase-independent mechanism.
Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody.
The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway.
Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma.
Oncogenic aberrations of p16INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control.
The PRAD-1/cyclin D1 oncogene product accumulates aberrantly in a subset of colorectal carcinomas.
Qin JJ, Wang W, Sarkar S, Voruganti S, Agarwal R, Zhang R
Oncotarget 2016 May 31;7(22):32566-78
Oncotarget 2016 May 31;7(22):32566-78
New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress.
Ercilla A, Llopis A, Feu S, Aranda S, Ernfors P, Freire R, Agell N
Nucleic acids research 2016 Jun 2;44(10):4745-62
Nucleic acids research 2016 Jun 2;44(10):4745-62
Serum vitamin D (25OHD3) levels and the risk of different subtypes of breast cancer: A nested case-control study.
Shirazi L, Almquist M, Borgquist S, Malm J, Manjer J
Breast (Edinburgh, Scotland) 2016 Aug;28:184-90
Breast (Edinburgh, Scotland) 2016 Aug;28:184-90
Identification of thyroid tumor cell vulnerabilities through a siRNA-based functional screening.
Anania M, Gasparri F, Cetti E, Fraietta I, Todoerti K, Miranda C, Mazzoni M, Re C, Colombo R, Ukmar G, Camisasca S, Pagliardini S, Pierotti M, Neri A, Galvani A, Greco A
Oncotarget 2015 Oct 27;6(33):34629-48
Oncotarget 2015 Oct 27;6(33):34629-48
Estrogen Receptor β Agonists Differentially Affect the Growth of Human Melanoma Cell Lines.
Marzagalli M, Casati L, Moretti RM, Montagnani Marelli M, Limonta P
PloS one 2015 Jul 30;10(7):e0134396
PloS one 2015 Jul 30;10(7):e0134396
Characterization of a new small bowel adenocarcinoma cell line and screening of anti-cancer drug against small bowel adenocarcinoma.
Suzuki H, Hirata Y, Suzuki N, Ihara S, Sakitani K, Kobayashi Y, Kinoshita H, Hayakawa Y, Yamada A, Watabe H, Tateishi K, Ikenoue T, Yamaji Y, Koike K
The American journal of pathology 2015 Feb;185(2):550-62
The American journal of pathology 2015 Feb;185(2):550-62
Expression and inactivation of glycogen synthase kinase 3 alpha/ beta and their association with the expression of cyclin D1 and p53 in oral squamous cell carcinoma progression.
Mishra R, Nagini S, Rana A
Molecular cancer 2015 Feb 3;14:20
Molecular cancer 2015 Feb 3;14:20
Acetylation-defective mutants of Pparγ are associated with decreased lipid synthesis in breast cancer cells
Tian L, Wang C, Hagen F, Gormley M, Addya S, Soccio R, Casimiro M, Zhou J, Powell M, Xu P, Deng H, Sauve A, Pestell R
Oncotarget 2014 September;5(17):7303-7315
Oncotarget 2014 September;5(17):7303-7315
Vitamin D signaling regulates oral keratinocyte proliferation in vitro and in vivo.
Yuan FN, Valiyaparambil J, Woods MC, Tran H, Pant R, Adams JS, Mallya SM
International journal of oncology 2014 May;44(5):1625-33
International journal of oncology 2014 May;44(5):1625-33
WNT signaling and distant metastasis in colon cancer through transcriptional activity of nuclear β-Catenin depend on active PI3K signaling.
Ormanns S, Neumann J, Horst D, Kirchner T, Jung A
Oncotarget 2014 May 30;5(10):2999-3011
Oncotarget 2014 May 30;5(10):2999-3011
SHIP2 signaling in normal and pathological situations: Its impact on cell proliferation.
Elong Edimo W, Schurmans S, Roger PP, Erneux C
Advances in biological regulation 2014 Jan;54:142-51
Advances in biological regulation 2014 Jan;54:142-51
Targeting cyclin-dependent kinase 1 (CDK1) but not CDK4/6 or CDK2 is selectively lethal to MYC-dependent human breast cancer cells.
Kang J, Sergio CM, Sutherland RL, Musgrove EA
BMC cancer 2014 Jan 20;14:32
BMC cancer 2014 Jan 20;14:32
Cyclin D1/cyclin-dependent kinase 4 interacts with filamin A and affects the migration and invasion potential of breast cancer cells.
Zhong Z, Yeow WS, Zou C, Wassell R, Wang C, Pestell RG, Quong JN, Quong AA
Cancer research 2010 Mar 1;70(5):2105-14
Cancer research 2010 Mar 1;70(5):2105-14
Adhesion control of cyclin D1 and p27Kip1 levels is deregulated in melanoma cells through BRAF-MEK-ERK signaling.
Bhatt KV, Spofford LS, Aram G, McMullen M, Pumiglia K, Aplin AE
Oncogene 2005 May 12;24(21):3459-71
Oncogene 2005 May 12;24(21):3459-71
Epidermal growth factor receptor and cyclin D1 are independently amplified and overexpressed in esophageal squamous cell carcinoma.
Sunpaweravong P, Sunpaweravong S, Puttawibul P, Mitarnun W, Zeng C, Barón AE, Franklin W, Said S, Varella-Garcia M
Journal of cancer research and clinical oncology 2005 Feb;131(2):111-9
Journal of cancer research and clinical oncology 2005 Feb;131(2):111-9
Cyclin D1 represses p300 transactivation through a cyclin-dependent kinase-independent mechanism.
Fu M, Wang C, Rao M, Wu X, Bouras T, Zhang X, Li Z, Jiao X, Yang J, Li A, Perkins ND, Thimmapaya B, Kung AL, Munoz A, Giordano A, Lisanti MP, Pestell RG
The Journal of biological chemistry 2005 Aug 19;280(33):29728-42
The Journal of biological chemistry 2005 Aug 19;280(33):29728-42
Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody.
Cheuk W, Wong KO, Wong CS, Chan JK
The American journal of surgical pathology 2004 Jun;28(6):801-7
The American journal of surgical pathology 2004 Jun;28(6):801-7
The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway.
Shtutman M, Zhurinsky J, Simcha I, Albanese C, D'Amico M, Pestell R, Ben-Ze'ev A
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5522-7
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5522-7
Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma.
de Boer CJ, Schuuring E, Dreef E, Peters G, Bartek J, Kluin PM, van Krieken JH
Blood 1995 Oct 1;86(7):2715-23
Blood 1995 Oct 1;86(7):2715-23
Oncogenic aberrations of p16INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control.
Lukas J, Aagaard L, Strauss M, Bartek J
Cancer research 1995 Nov 1;55(21):4818-23
Cancer research 1995 Nov 1;55(21):4818-23
The PRAD-1/cyclin D1 oncogene product accumulates aberrantly in a subset of colorectal carcinomas.
Bartkova J, Lukas J, Strauss M, Bartek J
International journal of cancer 1994 Aug 15;58(4):568-73
International journal of cancer 1994 Aug 15;58(4):568-73
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human and mouse samples with DCS-6 mAb.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 antibody PE conjugate (V2006PE)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 antibody PE conjugate (V2006PE)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (DCS-6). Confirmation of Integrity and Purity of the Antibody.
